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Allergenicity Prediction: FAO/WHO method

A report from a Joint FAO/WHO Expert Consultation on Foods Derived from Biotechnology proposed that cross-reactivity between a query protein and a known allergen has to be considered when there is 1) more than 35% identity in the amino acid sequence of the expressed protein, using a window of 80 amino acids and a suitable gap penalty, or 2) identity of 6 contiguous amino acids.
Using the FAO/WHO allergenicity prediction in our site, you can analyze the potential allergenicity of your query protein according to the two criteria above.
It should be noted that the prediction results will not necessarily indicate that your query protein has an allergenicity

Download example Excel
Upload a Excel file:

or Enter your protein sequence.

Do a full fasta alignment

Identity % or more , out of window size : AA or more
Expect value for FASTA Program :

Look for a small exact wordmatch

Identity contiguous AA

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